© Benaki Phytopathological Institute
Abstracts - 16th Hellenic Phytopathological Congress
67
pressive results were obtained by combin-
ing all of the (11) rhizobacteria with
S. rolfsii
,
or the combination of the 5 strains tested for
the effect of volatiles on the same fungus. In
almost all treatments mycelial growth was
very limited or non-existent, and an inabil-
ity of sclerotia germination to hyphae was
observed. Treatments of
S. rolfsii
with PiHa5-
II and S47 were the only exceptions in which
the inhibition zone was limited. The effect
of volatiles was also apparent, as the strains
PiHa5-II, ToZa7, ΤοΖa4-8 and ΤοΖα9-12 pre-
vented mycelial growth and germination
of sclerotia of
S. rolfsii
at a distance
,
where-
as
S76 showed different results in 3 replica-
tions. Finally, combination of
V. dahliae
and
S52 gave interesting results with fungal
growth being limited, and the formation of
microsclerotia inhibited.
Evaluation of Greek Trichoderma isolates effect on plant growth
characteristics of aronia (
Aronia melanocarpa
) plants
G.A. B
ARDAS
, P. P
ENTSOU
, M. D
OTSIOU
, H. M
ANDRAS
, G. M
OUTOULI
, A. T
SANAKTSIDOU
G.
P
ALATOS
and S. S
TEFANOU
Alexander Technological Educational Institution of Thessaloniki, GR-574 00 Sindos,
Thessaloniki, Greece
Aronia melanocarpa
fruit production is a
novel crop in Greek agriculture and research
is needed in order to define and optimize
the cultivation techniques in biological pro-
duction systems. The present research was
focused on the evaluation of three
Tricho-
derma
sp. isolates, belonging to
Trichoderma
harzianum
and
Trichoderma asperellum
spe-
cies, regarding their effect on plant growth
characteristics of aronia plants. All three
Trichoderma
sp. isolates, when applied sep-
arately, colonized aronia roots to the same
extent, with colonization levels ranging on
average from 58% at 2 days after treatment
to 94% at 30 d.a.t. However, the combined
treatment resulted in asignificant increase
in the total number of colonized roots com-
pared with the single treatments, with 80,
87, 94 and 95% root colonization observed
at, 2, 7, 15 and 30 d.a.t., respectively. In ad-
dition, the combined treatment was superi-
or with regard to all the growth characteris-
tics tested. Data from the single treatments
showed that plants treatedwith
Trichoderma
harzianum
isolates B2 or B3 had higher dry
root weights and numbers of leaves com-
pared to the untreated rootstocks, while
T.
asperellum
B1 treatment was better than the
control with regard to dry root and shoot
weight. In conclusion, specific biocontrol
isolates can form a complementary treat-
ment to assist
Aronia melanocarpa
commer-
cial fruit production in Greece.
Molecular identification of epiphytic yeasts isolated from grapevines and
their assessment as biological agents to control
Aspergillus
spp.
Ι.S. P
ANTELIDES
, O. C
HRISTOU
, D. T
SALTAS
and Ν. I
OANNOU
Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus
University of Technology, 3603 Limassol, Cyprus
The aim of this study was the isolation and
molecular identification of epiphytic yeasts
fromgrapevines and their assessment as bio-
logical agents against black aspergilli (
Asper-
gillus
section
Nigri
). Initially, epiphytic yeasts
were isolated from grapes of the varieties
‘Maratheftiko’ and ‘Cabernet Sauvignon’
originating from four areas of the Limassol
district during 2010. Genomic DNA was iso-
lated from each yeast and was used for the
amplification and sequencing of the D2 re-
gion of the nuclear large-subunit (LSU) ribo-
