Page 20 - Volume 2, Issue 1, January 2009
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Anagnostopoulos & Miliadis
20
© Benaki Phytopathological Institute
and diseases in cereals and included in the
European Commission Proficiency test 2007
for cereals were studied. The extraction pro-
cedure was based on liquid extraction with
acetone followed by dichloromethane and
petroleum ether. Water was added in the
sample before the extraction for better per-
formance. The method is simple, fast and
suitable for routine analysis and with the
same extraction method fruits, vegetables
and cereals are analyzed. The validation of
the method resulted in good accuracy with
recoveries of 66.5 – 120% and precision with
RSD of 0.7 – 8.9% and sensitivity meeting in
most cases the EU legislation requirements
for the detection limits. Prompted by the
satisfactory performance of the method, we
aim at further testing it for the determina-
tion of more active compounds of the same
chemical classes.
Literature Cited
Bempelou, E.D. and Liapis, K.S. 2006. Validation
1.
of a multi-residue method for the determina-
tion of pesticide residues in apples by gas chro-
Table 2.
Average recovery values and relative standard deviation for the 28 pesticides as de-
rived from the fortification experiments (LOQ
m
= Limit of Quantification of the method).
Analyte
Detector
LOQ
m
10×LOQ
m
(n=6)
(n=6)
C
( mg/Kg)
Recovery
(%)
RSD
(%)
C
( mg/Kg)
Recovery
(%)
RSD
(%)
Bifenthrin
ECD 0.045
100.1
6.4
0.45
101.9
3.8
Carbaryl
NPD 0.75
99.2
2.5
7.5
91.4
2.4
Chlorpyriphos
ECD 0.015
104.9
3.7
0.15
102.4
2.1
Chlorpyriphos methyl
NPD 0.075
103.7
3.4
0.75
86.4
2.6
Cyhalothrin-λ
ECD 0.03
90.4
4.9
0.3
116
2.6
Deltamethrin
ECD 0.075
96.9
4.4
0.75
119.9
2.5
Diazinon
ECD 0.03
94.7
2.6
0.3
118.3
0.7
Endosulfan-a
ECD 0.0075
92.9
8.9
0.075
106.8
2.0
Endosulfan-b
ECD 0.0075
66.5
6.8
0.075
107.3
1.9
Fenpropimorph
NPD
0.5
83.2
2.8
5
104.4
1.8
Imazalil
NPD 0.15
119.9
1.5
1.5
112.8
8.1
Iprodione
ECD 0.03
91.0
6.0
0.3
107.9
2.5
Kresoxim-methyl
ECD 0.075
100.5
4.7
0.75
102.0
2.8
Lindane
ECD 0.015
99.5
5.8
0.15
100.9
7.5
Malathion
NPD 0.375
103.9
5.8
3.75
106.1
7.5
Methacrifos
NPD 0.05
89.3
5.2
0.5
96.5
6.2
Parathion
ECD 0.03
95.4
4.6
0.3
119.1
1.7
Penconazole
ECD 0.03
98.2
5.1
0.3
100.1
1.5
Pirimicarb
NPD 0.15
100.8
2.8
1.5
88.5
2.1
Pirimiphos methyl
NPD 0.15
106.3
4.0
1.5
106.9
7.5
Procloraz
NPD
0.5
101.1
4.5
5
119.5
2.6
Procymidone
ECD 0.075
110.3
2.2
0.75
101.0
1.6
Propiconazole
NPD 0.075
100.8
4.1
0.75
114.1
0.8
Thiabendazole
NPD 0.75
112.4
3.7
7.5
88.2
2.7
Triadimefon
ECD 0.075
83.6
8.5
0.75
106.5
1.3
Triadimenol
ECD 0.075
100.9
5.0
0.75
119.8
1.9
Triazophos
NPD 0.15
113.3
4.3
1.5
114.3
4.4
Vinclozolin
ECD 0.075
104.3
3
0.75
100
1.7
