Page 18 - Volume 2, Issue 1, January 2009
Basic HTML Version
Anagnostopoulos & Miliadis
18
© Benaki Phytopathological Institute
The attained LOQ
m
values are shown in
Table 2. The lowest calculated LOQ
m
value
was 0.01 mg/kg for the analyte endosulfan
and the highest 0.75 mg/kg for the analyte
carbaryl.
As shown in Table 2, relative standard
deviation values (RSD) at the LOQ
m
level
were 1.5 - 8.9% and at the 10×LOQ
m
level 0.7
– 8.1%. These results indicate satisfactory
precision of the method.
In the 2007 Proficiency Test for cereals,
organized by the European Commission Ref-
erence Laboratories on Cereals and Feeding-
stuff and Single Residue Methods, the above
described method was applied in our labo-
ratory and the following results were ob-
tained: From the 28 pesticides that were val-
idated deltamethrin, diazinon, pirimiphos
methyl and propiconazole were detected in
the sample of the proficiency test. All 4 com-
pounds were determined with acceptable
z score values, ranging between –0.2 and
1.2, verifying the acceptable accuracy of the
method.
In conclusion in this study, 28 active in-
gredients of plant protection products of
various physicochemical characteristics and
chemical classes used for the control of pests
Table 1.
Retention times (R.T.), E.U. maximum residue level (MRL), minimum required perfor-
mance level (MRPL) of the organizer and limit of quantification of the instrument (LOQ
i
) of
the 28 pesticides.
Pesticides
R.T. (min)
E.U. MRL
(mg/kg)
MRPL
(mg/kg)
LOQ
i
(mg/kg)
DB-5MS
DB-17MS
Bifenthrin
26.7
0.5
0.05
0.03
Carbaryl
16.6
0.5
0.05
0.1
Chlorpyriphos
12.4
15.7
0.05
0.05
0.002
Chlorpyriphos methyl
10.5
13.9
0.05
0.05
0.003
Cyhalothrin-λ
30.4-31.4
33.8-34.1
0.02
0.02
0.004
Deltamethrin
37.1-37.4
1
0.05
0.003
Diazinon
9
10.5
0.02
0.02
0.006
Endosulfan-α
15.5
0.05
0.05
0.004
Endosulfan-β
18.7
0.05
0.05
0.004
Fenpropimorph
12.26
0.5
0.05
0.003
Imazalil
16.6
22.5
0.02
0.02
0.10
Iprodione
19.4-25.4
33.2
0.5
0.05
0.02
Kresoxim-methyl
18.2
26.2
0.05
0.05
0.025
Lindane
8.6
0.01
0.01
0.002
Malathion
11.9
16.2
8
0.05
0.03
Methacrifos
6.045
6.8
0.05
0.05
0.02
Parathion
12.4
16.4
0.05
0.05
0.006
Penconazole
13.9
18.3
0.05
0.05
0.006
Pirimicarb
11.6
13.6
0.05
0.05
0.05
Pirimiphos methyl
11.6
14.9
5
0.05
0.03
Procloraz
36.8
33.8
0.5
0.05
0.12
Procymidone
14.7
19.4
0.02
0.02
0.02
Propiconazole
22-22.4
29.2-29.5
0.05
0.05
0.07
Thiabendazole
23.6
0.05
0.05
0.37
Triadimefon
12.5
15.6
0.2
0.02
0.005
Triadimenol
14.4-14.7
18.4-19
0.2
0.05
0.03
Triazophos
32.4
0.2
0.1
0.02
Vinclozolin
10.4
12.7
0.05
0.1
0.006
