© Benaki Phytopathological Institute
Hellenic Plant Protection Journal - Special Issue
82
and low resistance levels to isopyrazam,
fenfuram and carboxin, but retained the
wild-type sensitivity to fluopyram, bixafen,
fluxapyroxad and benodanil. Similarly, the
H272Y showed moderate levels of resistance
to boscalid, and very levels of resistance to
isopyrazam, bixafen, fenfuram and carboxin,
but showed an increased sensitivity to ben-
odanil and fluopyram (negative cross-resis-
tance). The findings of the current research
can be useful both for future sensitivity
monitoring programmes and in the imple-
mentation of anti-resistance strategies.
Resistance of
Botrytis cinerea
populations from strawberry and
greenhouse-grown tomato to botryticides
P. K
ALOGEROPOULOU
, T. V
ELOUKAS
and G.S. K
ARAOGLANIDIS
Plant Pathology Laboratory, Faculty of Agriculture, Aristotles University of
Thessaloniki, GR-541 24 Thessaloniki, Greece
Botrytis cinerea
is a high risk pathogen for
fungicide resistance development and dur-
ing the past has presented the problem of
fungicide resistance development world-
wide. The present study was conducted to
investigate the fungicide sensitivity pro-
file of pathogen isolates obtained from
strawberry (from the Pieria and Ilia regions)
and greenhouse-grown tomato (regions
of Preveza, Kyparissia, and on Crete). In to-
tal 1,160 single spore isolates of the patho-
gen were tested for sensitivity to the SDHI
fungicide boscalid, the QoI pyraclostrob-
in, the anilinopyrimidine cyprodinil, the hy-
droxyanilide fenhexamid, the phenylpyrrole
fludioxonil, and the benzimidazole car-
bendazim. The isolates were characterized
as either sensitive or resistant to each of the
fungicides using a bioassay technique with
distinct discriminatory fungicide doses. The
isolates were grouped based on their resis-
tance phenotype profile. Results showed
that in fungal populations obtained from
green-house grown tomatoes on the island
of Crete, the predominant phenotype was
that of double resistance to benzimidazole
and anilinopyrimidine fungicides (48.9% of
the population), in the region of Preveza the
predominant phenotype was that of single
resistance to benzimidazoles (57.4% of the
population), while in the Kyparissia region
the sensitive isolates dominated the pop-
ulation (57.4%). In contrast, within the pop-
ulations obtained from strawberry fruits, a
high proportion of isolates (57%) exhibited
multiple resistance to anilinopyrimidines,
benzimidazoles, QoIs and SDHIs. None of
the isolates tested was found to be resis-
tant to hydoxyanilide or phenylpyrrole fun-
gicides. The results revealed a widespread
prevalence of boscalid resistant pheno-
types in the strawberry population rang-
ing from 46.7% to 76.8% in the Pieria and
Ilia regions, respectively. The resistance fre-
quency in the tomato population was signif-
icantly lower, with boscalid-resistance fre-
quency values of 0.8, 9.8 and 13.2% in the
Crete, Messinia and Preveza populations, re-
spectively. In addition, in SDHI-resistant iso-
lates the
sdh
B mutations associated with re-
sistance were identified using a PIRA-PCR
technique, which showed that H272R was
the predominant mutation in all of the sam-
pled populations.
Fitness and competitive ability of
Botrytis cinerea
strains possessing
different mutations in the
sdh
B gene
T. V
ELOUKAS
1
, P. K
ALOGEROPOULOU
1
, Α. Μ
ARKOGLOU
2
and G.S. K
ARAOGLANIDIS
1
1
Aristotle University of Thessaloniki, Faculty of Agriculture, Plant Pathology
Laboratory, POB 269, GR-541 24 Thessaloniki, Greece.
2
Agricultural University of
Athens, Laboratory of Pesticide Science, 75 Iera Odos Str., GR-118 55 Athens, Greece
