© Benaki Phytopathological Institute
Michaelakis
et al.
38
mortality was regressed against log
10
–trans-
formed dose; LC
50
, LC
90
values, and slopes
were calculated (SPSS 11.0).
Results
Chemical Composition of the Essential
Oils
Yields and chemical composition of the
obtained essential oils are shown in Table 1.
Literature data suggests that
M. pulegium
is
a chemical polymorph species both in qual-
itative and/or quantitative composition. Re-
cently, it has been demonstrated the link be-
tween the oil composition and origin of 38
populations of
M. pulegium
scattered along
Greece (12). Thus, the three different sam-
ples resulted in different chemical profiles.
In detailed, in EOpul1 pulegone (61.1%) was
Table 1.
Percentage composition of essential oils isolated from
M. pulegium
(three popula-
tions: EOpul1, EOpul2 and EOpul3),
M. spicata
(EOsp) and
M. piperita
(EOpip). Compounds are
listed in order of elution from an HP-5 MS column.
Components
Composition (%)
EOpul1
c
EOpul2
c
EOpul3
EOsp
EOpip
c
3-methyl cyclohexanone
a
0.4
α
-pinene
a
0.2
0.4
0.7
β
-pinene
a
0.2
0.2
1.2
3-octanol
a
1.4
0.4
0.7
0.2
limonene
a
1.2
2.7
1.8
eucalyptol
a
9.0
6.9
γ-terpinene
a
0.4
0.7
menthone
a*
4.3
1.8
1.6
39.0
isomenthone
a*
13.0
0.3
24.8
9.9
menthol
a*
25.9
terpinen-4-ol
a
0.7
cis-dihydro carvone
b
1.0
isopulegone
b
2.9
trans
-carveol
b
0.8
pulegone
a*
61.1
2.0
carvone
a*
71.8
piperitone
a*
1.1
92.6
69.3
1.3
piperitenone
a*
4.0
0.1
0.4
piperitenone oxide
b
1.8
β
-bourbonene
b
0.1
0.8
0.2
1.5
0.4
β
-elemene
b
1.1
0.2
β
-caryophyllene
a
2.2
3.5
β
-farnesene
a
0.1
0.6
germacrene D
b
0.1
1.3
2.9
bicyclogermacrene
b
0.8
0.5
caryophyllene oxide
a
0.2
Total
oil yield (ml/100 dry wt)
91.4
2.2
98.7
2.1
99.2
2.1
91.7
1.8
95.7
3.2
a
Comparison with pure standards.
b
Tentative identification based on data obtained from NIST 98 and Wiley 275 library of the GC-MS system and from
Adams CD computer library (16).
c
For these essential oils the phytochemical analysis for major ingredients (>1%) has already been published
(14).
* Components that were isolated and applied in bioassays.
1,2,3,4,5,6,7,8,9 11,12,13,14,15,16,17,18,19,20,...46